Identification of Macromolecule

Macromolecules contain more than one molecules. They are usually found in foods. For example, lipids, nucleic acids, carbohydrates and proteins are the living organisms that macromolecules are commonly found. Their functions are mostly storing energy and information. By performing this lab, types of macromolecules are found. There are three different experiments that we performed. They are Iodine test, Biuret Test and Benedict’s test. With eleven solutions includes one unknown solution, the macromolecules will be found in the solutions and also, the unknown solution will be identified after the experiments. In this case, unknown solution is unknown number

For example, from Iodine test, if the solution contains glycogen molecule, pale yellow iodine solution turns red-brown. If the solution contains starch molecule, the solution will turn blue black colour. For the Benedict’s test, blue coloured solution means reducing sugars and coloured precipitate means they contain some macromolecules. The colours they represent are yellow, green, red and brown. For the Biuret Test, the colour change occurs when there is protein inside the solution. Colour change will be violet or yellow.

Materials and Methods

Materials we used were
12 small disposable plastic beakers
Pasteur pipettes & bulbs
1 spot-plate
test-tube rack
12 test-tubes
1 test-tube clamp
1 dropper bottle containing 1% copper sulfate
1 dropper bottle containing iodine solution
1 large beaker with boiling chips
1 marker
15 ml of 1% glucose solution
15ml of 0.3% glucose-1-phosphate
15ml of 1% maltose solution
15ml of honey solution
15ml of 1% sucrose solution
15ml of 1% lactose solution
15ml of 1% glycogen solution
15ml of 1% starch solution
15ml of protein, 15ml of beer
15ml of distilled water
and 15ml of #210 unknown solution
10% Sodium hydroxide
1% copper sulfate.

Methods – Three experiments were performed in order, Iodine test, Benedict’s test and Biuret Test. Before the experiments are begun, put all the 15ml of solutions into the beaker #1 to #12. These beakers are numbered with marker. Tube #1 is filled to with the approximately 1 ml of the liquid from beaker #1 (1/2 of the full Pasteur pipette is approximately 1 ml). Tube #2 is filled with the approximately 1 ml of the liquid from beaker #2. Untill all 12 tubes are filled up with 1 ml of the appropriate solution. 2ml of Benedict’s solution is added to each tube. The contents are mixed to each tube thoroughly. Boil the contents of each tube by placing the tube in a boiling water bath which has boiling chips in the water for about 5 minutes and note the colour change. Finally.

All of the observations are recorded and explained. Firstly, for the Iodine test, 1 drop of the solution is put from the beaker #1 into a clean well on a spot-plate. Then one drop of iodine solution is added to the sample drop. This is how iodine test is done. Any colour change is recorded, as well as any differences and other observations. Iodine test is repeated on every other remaining solutions. All the observations are recorded on a piece of paper. Benedict’s test was done with five procedures. Firstly, the tubes were Observation – before and after colour, post or negat, beaker # and solu name Discussion – explain everythign in detail fuck