Factors that affect enzyme activity

Since the active site for all molecules of one enzyme will be made up of the same arrangement of amino acids, It has a highly specific shape. Generally, there is only one active site on each enzyme molecule and only one type of substrate molecule will fit into it. This specificity leads to the lock and key hypothesis, Source 1: http:/twwa. s-cool. co. uk/a-level/blology/ biological-molecules-and-enzymes/revise-it/enzymes Source 2: http://cllck4blology. lnfo/c4b?/chem3. 6. htm#one a) Large globular protein enzyme b) Active Site where the substrate combines to the enzyme ) Substrate which fits the active site d) Activated complex.

The substrate is weakened to allow the reaction. e) Unchanged enzyme/ re-used at low concentration f) Product of the reaction In my investigation, I will be using the enzyme catalase, which is found in most living organisms. It catalyses the decomposition of hydrogen peroxide Into water and oxygen. 2H202 + catalase > 2820 + 02 Catalase dramatically reduces the activation energy needed for the reaction. Without catalase the decomposition would take much longer, and would not be fast enough to sustain human life.

Hydrogen peroxide is also a dangerous, very potent by-product of metabolism, so it is essential that it is broken down quickly, otherwise it would cause damage to cells. The activity of an enzyme is affected by its environmental conditions. Changing these will alter the rate of reaction caused by the enzyme. In nature, organisms adjust the conditions of their enzymes to produce an optimum rate of reaction, where necessary, or they may have enzymes which are adapted to function well In extreme conditions where they live.

Enzyme Concentration: at low enzyme concentration there is great competition for he active sites and the rate of reaction is low. As the enzyme concentration increases, there are more active sites and the reaction can proceed at a faster rate, for more enzymes will be colliding with substrate molecules. Eventually, increasing tne enzyme concentratlon Deyona a certain polnt nas no erect Decause tne suDstrate concentration becomes the limiting factor.

Inquiring upon this factor, it is obvious to anticipate increasing enzyme concentration will also increase rate of reaction based upon contextual knowledge and after casting a certain amount of enzyme oncentration, it will no longer be the limiting factor. If I experiment on this factor, I would perceive my data to resemble the graph below, as it exemplifies how increasing enzyme concentration increases rate of reaction(shown through line climbing) until it becomes the limiting factor and the rate of reaction does not increase(shown through line not climbing).

Source 3 :http://alevelnotes. com/Factors- affecting-Enzyme-Activity/146 Substrate Concentration: similar to the enzyme concentration, at low concentrations of substrate there is a low rate of reaction. This is because there are few substrate molecules to react with active sites and therefore limiting the number of reactions happening. Henceforth, increasing the substrate concentration will increase the rate of reaction. This is because more substrate molecules will be colliding with enzyme molecules, so more product will be formed.

However, after a certain concentration, any increase will have no effect on the rate of reaction, since Substrate Concentration will no longer be the limiting factor. The enzymes will effectively become saturated, and will be working at their maximum possible rate. If I was to investigate this factor, I would predict the rate of reaction will increase as substrate concentration increases, until a certain concentration is added when the substrate molecules are in excess resulting in enzyme saturation.

The graph (below) demonstrates my prediction. Source 3 :http://alevelnotes. com/Factors-affecting-Enzyme-Activity/146 enzyme and substrate Simple image portraying proposed image for concentration (discussed in according factors). Mentioned as “picture of proposed investigation below’ Temperature: All enzymes ave optimal temperatures, the temperature at which an enzyme produces the highest reaction rate for a specific reaction. The majority of enzymes in the human body works best at 37 Celsius degrees.

This is because 37 degrees Celsius is the bodys internal temperature and enzymes such as catalase, have been adapted to work best at that certain temperature. Below the optimum temperature, substrates have little kinetic energy and fewer enter the active site to be catalyzed. However, as temperature increases towards the optimum, the substrates and enzymes gain more inetic energy and collide more often leading to a chemical reaction. When the temperature goes above the optimum, the bonds holding enzymes together also gain kinetic energy, increasing the speed at which they vibrate.

This leads to the bonds breaking within the enzyme, making it change shape. This change in shape means that the active site is less complementary to the shape of the substrate, so that it is less likely to catalyse the reaction. Eventually, the enzyme will Decome denatured ana will no longer Tunctlon. I nen as temperature Increases more nzymes’ molecules’ active sites will become less complementary for the substrate molecules and then more enzymes will be denatured.

This will decrease the rate of reaction. If I examined this factor, I would predict that the rate of reaction will peak at 37 degrees Celsius, as that is the optimum temperature of catalase. Also, as stated in the latter paragraph, increasing or decreasing the temperature from its optimum will lower the rate of reaction. Therefore, I should expect the data I collected to be similar of the graph below. Source 4: http://www. rsc. rg/Education/Teachers/Resources/cfb/ enzymes. tm PH: pH measures the acidity and basicity ofa solution. It is a measure of the hydrogen ion (H+) concentration, and therefore a good indicator of the hydroxide ion (OH-) concentration. It ranges from PHI to pH14. Lower pH values mean higher H + concentrations and lower OH- concentrations. Unlike the same optimal temperature for all enzymes that dwell in the human body (370c); the optimum pH varies for the enzymes. For example, the enzyme pepsin has an optimum pH of 2. 0 whereas catalase has an optimum of 7. 6.

Enzymes in different locations have different Optimum pH values since their environmental conditions may be different. In this instance, pepsin operates most competently at pH 2 because it is commonly found in the stomach, where pH is low due to the presence of hydrochloric acid. Enzymes work in small ranges of pH values, so any change above or below the optimum will cause a sudden decrease in rate of reaction, since more of the enzyme molecules will have active sites whose shapes are not (or at least are less) complementary to the shape of their substrate.

Small changes below or above the optimum, does not cause a permanent change to the enzymes since the bonds can be reformed. However, extreme changes in pH can cause enzymes to denature and permanently loose their function. When the pH is changed from the optimal of the certain enzyme, the H+ and OH- interfere with hydrogen and ionic bonds that hold together an enzyme, since they will be attracted or repelled by the charges created by the bonds. This interference causes a change in the shape of the enzyme and most importantly, the active site.

Calculate the price
Make an order in advance and get the best price
Pages (550 words)
$0.00
*Price with a welcome 15% discount applied.
Pro tip: If you want to save more money and pay the lowest price, you need to set a more extended deadline.
We know how difficult it is to be a student these days. That's why our prices are one of the most affordable on the market, and there are no hidden fees.

Instead, we offer bonuses, discounts, and free services to make your experience outstanding.
How it works
Receive a 100% original paper that will pass Turnitin from a top essay writing service
step 1
Upload your instructions
Fill out the order form and provide paper details. You can even attach screenshots or add additional instructions later. If something is not clear or missing, the writer will contact you for clarification.
Pro service tips
How to get the most out of your experience with MyStudyWriters
One writer throughout the entire course
If you like the writer, you can hire them again. Just copy & paste their ID on the order form ("Preferred Writer's ID" field). This way, your vocabulary will be uniform, and the writer will be aware of your needs.
The same paper from different writers
You can order essay or any other work from two different writers to choose the best one or give another version to a friend. This can be done through the add-on "Same paper from another writer."
Copy of sources used by the writer
Our college essay writers work with ScienceDirect and other databases. They can send you articles or materials used in PDF or through screenshots. Just tick the "Copy of sources" field on the order form.
Testimonials
See why 20k+ students have chosen us as their sole writing assistance provider
Check out the latest reviews and opinions submitted by real customers worldwide and make an informed decision.
Business and administrative studies
excellent job!
Customer 452773, May 25th, 2023
Business and administrative studies
always perfect work and always completed early
Customer 452773, February 21st, 2023
Business and administrative studies
excellent job
Customer 452773, March 12th, 2023
Nursing
I just need some minor alterations. Thanks.
Customer 452547, February 10th, 2021
Business and administrative studies
Perfect
Customer 452773, February 23rd, 2023
Nursing
Impressive writing
Customer 452547, February 6th, 2021
English 101
great summery in terms of the time given. it lacks a bit of clarity but otherwise perfect.
Customer 452747, June 9th, 2021
Business and administrative studies
excellent job thank you Your Score 166.25/ 175- A 1. Current Culture 15% of total grade 18.37 Criterion "1. Current Culture" has textual feedback Criterion Feedback I see interesting points, though, in general they are not about the culture.
Customer 452773, June 4th, 2023
LEADERSHIP
excellent job
Customer 452773, August 12th, 2023
FIN571
excellent
Customer 452773, March 15th, 2024
Philosophy
Thank you
Customer 452811, February 17th, 2024
business
Great job
Customer 452773, February 13th, 2023
11,595
Customer reviews in total
96%
Current satisfaction rate
3 pages
Average paper length
37%
Customers referred by a friend
OUR GIFT TO YOU
15% OFF your first order
Use a coupon FIRST15 and enjoy expert help with any task at the most affordable price.
Claim my 15% OFF Order in Chat
Close

Sometimes it is hard to do all the work on your own

Let us help you get a good grade on your paper. Get professional help and free up your time for more important courses. Let us handle your;

  • Dissertations and Thesis
  • Essays
  • All Assignments

  • Research papers
  • Terms Papers
  • Online Classes
Live ChatWhatsApp